CRISPR Systems Overview

We identified CRISPR array structures in 4,313 MAGs, with 657 MAGs containing Cas proteins, thereby forming complete CRISPR-Cas systems.

Notably, 57.5% of the Cas protein types are unclassified, with Type ID being the most common classified type. This indicates a high degree of novelty in the CRISPR systems found in Greenlandic soils.

We detected 10,286 spacers, and the median spacer length was 36 bp. Genomes of approximately 7.5 Mb had the highest number of spacers and Cas proteins.

When these spacers were aligned against the entire NCBI RefSeq database, only 146 (1.5%) spacers yielded significant hits. This suggests most spacer-target interactions are novel, possibly involving host-virus or host-mobile genetic element interactions that have not been previously characterized.

High Novelty Indicator The extremely low hit rate against known databases underscores the unique nature of the viral and mobile genetic element communities interacting with Greenlandic soil microbiomes.

Sunburst Diagram Visualization

Cas type numbers of CRISPR-Cas systems in MAGs
Phylogenetic tree of Greenlandic MAGs
An example of CRIPSR-Cas system in Greenlandic MAGs

CRISPR Systems Browser

Cas Proteins Details
Genome Cas ID Type Subtype Protein Start End Strand Sequence