These 5,307 MAGs represented 17.6±8.6% of the microbial communities in Greenlandic soils by coverage, in stark contrast to the 0.001%–2.8% recovery rate of MAGs from eight other studies focused on the Arctic and the global environments.
Furthermore, a comparison of Greenlandic MAGs to those from 11 other studies and NCBI databases revealed that 94.8% of the MAGs had a maximum average nucleotide identity (ANI) below 95%, indicating we uncovered a large proportion of potentially novel species (n=5270/5307), genera (n=1710/5307), families (n=303/5307) and order (n=53/5307) from the Greenlandic microbiome.
Phylogenetic analysis of the MAGs revealed extensive microbial diversity in Greenlandic soils, identifying 5,277 bacterial and 30 archaeal MAGs. The most abundant phyla were Actinobacteriota (23.0%), Proteobacteria (21.8%), and Acidobacteriota (17.0%).
Our study provided a phylogenetic gain of 48.8%, significantly expanding the representation of known taxa, particularly Eremibacterota and Dormibacterota, which were respectively 6.9 and 3.4 times greater than those in the Genome Taxonomy Database (GTDB) (Release 95).
| ID | Completeness (%) | Contamination (%) | Genome Size | GC_Content (%) | Quality | GTDB Classification | Contigs | tRNA | 5S rRNA | 16S rRNA | 23S rRNA | Total rRNA | NCBI Classification | Min Contig | Max Contig | Mean Contig | Median Contig | N50 |
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